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  1. Formulation and evaluation of sitagliptan floating tablets Download Article

    M. Sambasiva rao, A. Sunil kumar reddy, A. Ashok Kumar
    • Article Type: Research Article
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    • Pages (01-10)
    • No of Download = 134

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    Gastro retentive dosage form using Guar gum was prepared to develop floating tablets of Sitagliptin that could retain in the stomach for longer periods of time delivering the drug to the site of action, i.e., stomach. The pre-compression parameters of all formulations showed good flow properties and these can be used for tablet manufacture. The post-compression parameters of all formulations were determined and the values were found to be satisfactory. From the drug content and in-vitro dissolution studies of the formulations, it was concluded that the formulation F9 i.e. the formulation containing guargum, Sodium bicarbonate, citric acid, micro crystalline cellulose and Magnesium stearate  is the best formulation. As a result of this study it may be concluded that the floating tablets using a guar gum in optimized concentration can be used to increase the GRT of the dissolution fluid in the stomach to deliver the drug in a sustained manner. The concept of formulating floating tablets of Sitagliptin offers a suitable and practical approach in serving desired objectives of gastro retentive floating tablets.

  2. Formulation and evaluation of sumatriptan oral thin films Download Article

    M. Sambasiva Rao, A. Sunil Kumar Reddy, A. Ashok Kumar
    • Article Type: Research Article
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    • Pages (11-21)
    • No of Download = 212

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    The main objective of the study was to formulate and evaluate oral thin film containing Sumatriptan succinate. The 4 and 5 % w/v HPMC, PVA, CMC films were prepared by solvent casting method. Compatibility of Sumatriptan with polymers was confirmed by FT-IR studies. Films were evaluated for weight variation and thickness showed satisfactory results. Tensile strength and folding endurance of the films were increased with increase in the concentration of polymer due to increase in the elasticity nature of the polymer. Mouth dissolving time and disintegration time of the films were increased with increase in the concentration of the polymer, as more fluid is required to wet the film in the mouth. The presence of disintegrant showed a considerable effect on the disintegration time of the films. Content uniformity study showed that the drug is uniformly distributed in the film. No differences were observed in invitro dissolution of drug from the film I - VI as the film instantly gets wet by dissolution medium. Present study reveals that all the formulated films showed satisfactory film parameters. It can be concluded that, Oral thin film-containing Sumatriptan can be prepared by solvent casting method. 4% w/v of HPMC (FV) film exhibited required tensile strength, folding endurance and disintegration time. The drug release was about 98.5 % in 300 seconds. 

  3. New RP HPLC method for the simultaneous estimation of sulbactum and ceftriaxone in pharmaceutical dosage form Download Article

    M. Sambasiva Rao, A. Sunil Kumar Reddy, A. Ashok Kumar
    • Article Type: Research Article
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    • Pages (22-41)
    • No of Download = 160

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    A simple and selective LC method is described for the determination of Sulbactum and Ceftriaxone tablet dosage forms. Chromatographic separation was achieved on a C18 column using mobile phase consisting of a mixture of mixture of 60 volumes of 20mM Phosphate buffer pH 3.5: 40 volumes of Acetonitrile (60:40 v/v) with detection of 210 nm. Linearity was observed in the range 30-70 µg /ml for Sulbactum (r2 =0.9998) and 60-140µg /ml for Ceftriaxone (r2 =0.9983) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim. The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.

  4. New RP HPLC method for the simultaneous estimation of rosuvastatin and aspirin in pharmaceutical dosage form Download Article

    M. Sambasiva Rao, A. Sunil Kumar Reddy, A. Ashok Kumar
    • Article Type: Research Article
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    • Pages (42-63)
    • No of Download = 95

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    A simple and selective LC method is described for the determination of Rosuvastatin and Aspirin tablet dosage forms. Chromatographic separation was achieved on a C18 column using mobile phase consisting of A mixture of 60 volumes of 20mM Phosphate buffer pH 3.5: 20 volumes of Acetonitrile and 20 voulmes of Methanol. With detection of 232 nm. Linearity was observed in the range 12-28 µg /ml for Rosuvastatin (r2 =0.9977) and 90-210 µg /ml for Aspirin (r2 =0.9953) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim.  The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing % RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.

  5. Preparation and evaluation of deferasirox effervescent release tablets Download Article

    G. Ravali, Desani Ravali Reddy, Jaffer Sadik, Abdul Lateef
    • Article Type: Research Article
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    • Pages (64-71)
    • No of Download = 232

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    Deferasirox is an oral iron chelater used to reduce chronic iron over load in patients who are receiving long term blood transfusion for condition such as beta-thalassemia. In this study deferasirox drug were formulated by direct compression. Six formulations of effervescent tablets were prepared by using different concentrations of effervescent agents to get desired release profile of reference product. Drug - Exciepient compatibility was studied by FT-IR spectral analysis. Effervescent tablets of deferasirox drug were prepared by using various excipients .Pre compressive parameters like carr’s index of all formulations between 22.54 ± 0.1 to 11.68 ±0.19, indicates passable compressibility index. Angle of repose of formulations from 37.34±0.04 to 33.50 ±0.14 i.e.., it declares that all are possessing good flow properties and hausners ratio of all formulations was 1.29±0.09 to 1.12±0.10 which satisfies the limits of compressibility. Post compressive parameters like weight are within limits .Hardness test of all the formulations from 9.3± 0.13 to 10.3 ±0.45 kg/cm2 .All the evaluation parameters were under acceptable ranges. The in vitro drug dissolution studies were carried out for the formulations in pH6.8 phosphate buffer .Dissolution profiles of all trials were done among all the formulations F6 better release. Stability studies were carried out for optimized formulation as per ICH guidelines. 

  6. New RP HPLC method for the estimation of imatinib in pharmaceutical dosage form Download Article

    M. Sambasiva Rao, A. Sunil Kumar Reddy, A. Ashok Kumar
    • Article Type: Research Article
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    • Pages (72-94)
    • No of Download = 165

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    A simple and selective LC method is described for the determination of Imatinib dosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of Water: Acetonitrile (30:70 v/v), with detection of 272nm. Linearity was observed in the range 50-150 µg /ml for Imatinib (r2 =0.9976) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim. The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.

  7. Evaluation of wound healing activity of leaves of Mirabilis jalapa l in experimentally induced diabetic rats Download Article

    Mohammed Vazir, Bindhu K, Kalaikovan T, Ashok Subedi
    • Article Type: Research Article
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    • Pages (95-98)
    • No of Download = 391

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    To evaluate the wound healing activity of leaves of Mirabilis Jalapa L in experimentally induced diabetic rats using Excision wound model, Incision wound model, Dead space wound model. Healthy albino wistar rats (200-250gm) of either sex, were divided into three groups (n=6).Group I (control) receives plain drinking water. Group II (diabetic control-receives plain drinking water) and Group III (diabetic group- receives 1000mg/kg b.wt of Mirabilis Jalapa. In excision wound model percentage of wound contraction was measured on 3rd, 6th, 9th, 12th, 15th, 18th, 21st day. In Incision wound model the wound breaking strength was measured on the 10th day. In dead space wound model the wet and dry weights of granulation tissue was recorded from the animals after 10days of wounding. The hydroxyproline content was measuring absorbance at 540nm using colorimeter. The data was analyzed by one way ANOVA followed by Dunnet`s test. The values were expressed as Mean±SEM. **P<0.01 was considered as significant.  Mirabilis Jalapa showed significant increase in wound contraction, wound breaking strength, wet and dry weight of the tissue, and amount of hydroxyproline in extract treated groups of the animals. The Mirabilis Jalapa showed significant wound healing activity in an experimentally induced diabetic rat.

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