Articles

  1. A newly validated HPLC method development for simultaneous estimation of ritonavir and lopinavir Download Article

    Jeyabaskaran.M, Prof.Rambabu.C, Dr.Sree Janardhanan V, Lakshmi Maneka S and Hemanth Sairam P
    • Article Type: Research Article
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    • Pages (1-8)
    • No of Download = 290

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    The aim of the present work was to develop a isocratict RP-HPLC for simultaneous analysis of ritonavir and lopinavir in tablet dosage form. Method: chromatographic system was optimized using a Agilent XDB C18(150 x 4.6mm,5µm) column with potassium dihydrogen phosphate (pH 4.6) and acetonitrile in the ratio of 45;55, as a mobile phase, at a flow rate of 1.0 ml/min. detection was carried out at 215nm by a photodiode array detector. Result: ritonavir and lopinavir were eluted with retention times of 4.821 and 3.814mins respectively. Beer’s lambert’s law was obeyed over the concentration ranges of 12.5 to 50µg/ml and 50 to 200µg/ml for ritonavir and lopinavir, respectively. Conclusion: the high recovery and low coefficients of variation confirm the suitability of the method for simultaneous analysis of both drugs in a tablet dosage form. Statistical analysis proves that the method is sensitive and significant for the analysis of ritonavir and lopinavir in pure and in pharmaceutical dosage form without any interference from the excipients. The method was validated in accordance with ICH guidelines. Validation revealed the method is specific, rapid, accurate, precise, reliable, and reproducible.

  2. Formulation of an alpha Glucosidase Inhibitors Drug as Mucoadhesive Microspheres – A Review Download Article

    Piyali Dey, K Jesindha Beyatricks, D Agilandeswari, M K Mohan Maruga Raja, S Preethi , V Tincy
    • Article Type: Review Article
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    • Pages (9-14)
    • No of Download = 404

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    α- glucosidase inhibitors are oral anti-diabetic drugs. These drugs are competitive inhibitors of the intestinal α- glucosidases and reduce post meal excursions by delaying digestion and absorption of starch and disaccharides. Their mechanism of action being limited to the intestinal brush border membrane, and owing to their structural features, they have limited systemic bioavailability, due to which they have much reduced side-effect profile compared to contemporary anti hyperglycemic drugs. Therefore, in the present investigation, an attempt will be made to develop mucoadhesive microspheres of α-glucosidase inhibitor (Miglitol) to enhance the bio- availability and to further reduce the dose and frequency of administration. Microspheres form an important part of novel drug delivery systems. They have carried applications and are prepared using assorted polymers. However, the success of these microspheres is limited owing to their short residence time at the site of absorption. It would therefore be advantageous to have means for providing an intimate contact of the drug delivery system with the absorbing membranes. This can be achieved by coupling bioadhesion characteristics to microspheres and developing bioadhesive microspheres. Bioadhesive microspheres have advantages such as efficient absorption and enhanced bioavailability of drugs owing to a high surface-to-volume ratio a much more intimate contact with the mucus layer and specific targeting of drugs to the absorption site.

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